Application

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2 is an antibody against Green Fluorescent Protein for use in IP, WB & IC.

Immunocytochemistry

Immunoblotting: 1:1000

Immunoprecipitation

Optimal working dilutions and protocols must be determined by the end user.

Research Sub CategoryEpitope Tags

Research CategoryEpitope Tags & General Use

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Immunogen

highly-purified native GFP

Epitope: original GFP only

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.

Format: Purified

Quality

Tested

Specificity

Directed against a bacterially-expressed fusion protein. Also reacts with red-shifted GFP-variant. Does not react with enhanced GFP.

Storage and Stability

Store at 2-8°C for up to 12 months.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.